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rabbit anti asic2 (Alomone Labs)

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Alomone Labs rabbit anti asic2
Rabbit Anti Asic2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+asic2/Anti-ASIC2a+Antibody/pm31895269-95-20-23
Average 93 stars, based on 23 article reviews

rabbit anti asic2 - by Bioz Stars, 2026-10

93/100 stars

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Membrane:

Article Title: Acid-sensing ion channels contribute to the effect of acidosis on the function of dendritic cells.
Article Snippet: .. The membrane was blocked with 5% (w/v) nonfat milk in TBST for 2 h at 37 ̊C and then incubated with rabbit anti-ASIC1, rabbit anti-ASIC2, or rabbit anti-ASIC3 Abs (all from Alomone Laboratories), as well as mouse anti-actin Ab-5 Ab (BD Biosciences), which served as a loading control, diluted in 5% (w/v) nonfat milk in 0.1% TBST overnight at 4 ̊C. ..

Incubation:

Article Title: Extracellular acidosis modulates the endocytosis and maturation of macrophages.
Article Snippet: 0008-8749/$ see front matter 2013 Elsevier Inc. A http://dx.doi.org/10.1016/j.cellimm.2012.12.009 Abbreviations: APC, antigen presenting cell; ASIC, cluster of differentiation; cDNA, complementary DNA macrophage; DMEM, dulbecco’s modified eagle med acid; ELISA, enzyme linked immunosorbent assay channel/degenerin; FACS, fluorescence-activated cell FITC, fluorescein isothiocyanate; GAPDH, glyceralde nase; HEPES, 2-[4-(2-Hydroxyethyl)-1-piperazinyl]eth radish peroxidase; IgG, immunoglobulin G; IL, inte colony-stimulating factor; MFI, mean fluorescence i compability complex; NSAIDs, nonsteroid anti-inflamm buffered solution; PCR, polymerase chain reaction polyvinylidene fluoride; RNA, ribonucleic acid; RT polymerase chain reaction; TNF, tumor necrosis facto ⇑ Corresponding author.. Fax: +86 27 83693500.. E-mail address: tanzheng@mail.hust.edu.cn (Z. Tan Extracellular acidosis is involved in various pathological situations of central nervous system and the effects are largely mediated by acid sensing ion channels (ASICs).

Article Title: Epigenetic upregulation of acid-sensing ion channel 1 contributes to gastric hypersensitivity in adult offspring rats with prenatal maternal stress.
Article Snippet: Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou 215123, China.. Jiangsu Key Laboratory of Anesthesiology & Jiangsu Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou 221004, China.. The Hand Surgery Research Center, Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong 226001, China.

Control:

Labeling:

Cell-Signaling:

Image Search Results

Primers and bp product size used for genotyping, RT-PCR, and qPCR for ASIC1, ASIC2, ASIC3, β-actin, and RNA18S

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Primers and bp product size used for genotyping, RT-PCR, and qPCR for ASIC1, ASIC2, ASIC3, β-actin, and RNA18S

Article Snippet: Blots were blocked for 1 h with 5% milk then incubated for 48 h at 4°C with rabbit anti-ASIC1 (1:500, no. ab5674P; Millipore), rabbit anti-ASIC2 (1:500, no. ab5460; Millipore), or rabbit anti-ASIC3 (1:400, no. ab49333; Abcam).

Techniques: Sequencing

Acid-sensing ion channel (ASIC)2 and ASIC3 are expressed in pulmonary arterial smooth muscle cells (PASMC). Representative electrophoresis gel showing reverse transcriptase (RT)-PCR for ASIC2 (240 bp), ASIC3 (432 bp), and β-actin (294 bp) in PASMC from wild-type (WT), ASIC2−/−, and ASIC3−/− mice.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Acid-sensing ion channel (ASIC)2 and ASIC3 are expressed in pulmonary arterial smooth muscle cells (PASMC). Representative electrophoresis gel showing reverse transcriptase (RT)-PCR for ASIC2 (240 bp), ASIC3 (432 bp), and β-actin (294 bp) in PASMC from wild-type (WT), ASIC2−/−, and ASIC3−/− mice.

Techniques: Electrophoresis, Reverse Transcription Polymerase Chain Reaction

Average systemic MABP (mm Hg) and HR in beats/min in conscious WT, ASIC2 −/− , and ASIC3 −/− male mice by radiotelemetry recorded over 72 h

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Average systemic MABP (mm Hg) and HR in beats/min in conscious WT, ASIC2 −/− , and ASIC3 −/− male mice by radiotelemetry recorded over 72 h

Techniques:

Chronic hypoxia (CH) does not significantly alter acid-sensing ion channel (ASIC)2 or ASIC3 protein levels in intrapulmonary arteries. A: representative Western blots of ASIC2 (predicted ∼69 kDa), ASIC3 (predicted ∼59 kDa), and corresponding Coomassie-stained blot. All samples were collected at the same time and processed in parallel on 2 gels for each antibody to accommodate 12 samples [6 control (Con) and 6 CH] from male wild-type (WT) mice. B: summary data detecting ASIC2/3 expression (normalized to entire lane on Coomassie-stained blot) in isolated intrapulmonary arteries from Con and CH WT mice. Values are means ± SE; dots indicate n = 6/group, analyzed by unpaired t-test.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Chronic hypoxia (CH) does not significantly alter acid-sensing ion channel (ASIC)2 or ASIC3 protein levels in intrapulmonary arteries. A: representative Western blots of ASIC2 (predicted ∼69 kDa), ASIC3 (predicted ∼59 kDa), and corresponding Coomassie-stained blot. All samples were collected at the same time and processed in parallel on 2 gels for each antibody to accommodate 12 samples [6 control (Con) and 6 CH] from male wild-type (WT) mice. B: summary data detecting ASIC2/3 expression (normalized to entire lane on Coomassie-stained blot) in isolated intrapulmonary arteries from Con and CH WT mice. Values are means ± SE; dots indicate n = 6/group, analyzed by unpaired t-test.

Techniques: Western Blot, Staining, Expressing, Isolation

Right ventricular systolic pressure (RVSP) is augmented in ASIC2−/− mice. Summary data for RVSP (A) and ratio of right ventricular (RV) to left ventricular plus septum (LV + S) heart weight (B) in anesthetized wild-type (WT), acid-sensing ion channel (ASIC)2−/−, and ASIC3−/− mice following exposure control or chronic hypoxia (CH) conditions. Values are means ± SE; n/group are indicated at the bottom of the bars as no. of males/females and as color-coded dots: black circle, control male; red circle, control female; black square, CH male; red square, CH female. There is no significant difference between males and females (P = 0.62). ****P < 0.0001 vs. respective control (Con) and ####P < 0.0001 vs. corresponding WT, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple-comparisons test.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Right ventricular systolic pressure (RVSP) is augmented in ASIC2−/− mice. Summary data for RVSP (A) and ratio of right ventricular (RV) to left ventricular plus septum (LV + S) heart weight (B) in anesthetized wild-type (WT), acid-sensing ion channel (ASIC)2−/−, and ASIC3−/− mice following exposure control or chronic hypoxia (CH) conditions. Values are means ± SE; n/group are indicated at the bottom of the bars as no. of males/females and as color-coded dots: black circle, control male; red circle, control female; black square, CH male; red square, CH female. There is no significant difference between males and females (P = 0.62). ****P < 0.0001 vs. respective control (Con) and ####P < 0.0001 vs. corresponding WT, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple-comparisons test.

Techniques:

Mean BW (g), RVDP (mmHg), HR in beats/min, RV CI (1/s), and HCT (%red blood cells) in WT, ASIC2 −/− , and ASIC3 −/− mice exposed to Con or CH conditions

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Mean BW (g), RVDP (mmHg), HR in beats/min, RV CI (1/s), and HCT (%red blood cells) in WT, ASIC2 −/− , and ASIC3 −/− mice exposed to Con or CH conditions

Techniques:

Small pulmonary arterial muscularization is greater in acid-sensing ion channel (ASIC)2−/− compared with wild-type (WT) mice. A: representative smooth muscle α-actin immunofluorescence images of lung sections from control (Con) and chronic hypoxia (CH) WT, ASIC2−/−, and ASIC3−/− mice. Fluorescence images were digitally inverted to provide better contrast and visibility of immunofluorescence. B: %muscularization calculated as %thresholded smooth muscle α-actin area divided by total arterial wall area. All vessels were <100 µm. Values are means ± SE; n = no. of vessels (indicated in bar) analyzed from 4 animals/group. ****P ≤ 0.0001 vs. the control group; ####P < 0.0001 vs. corresponding WT mice.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Small pulmonary arterial muscularization is greater in acid-sensing ion channel (ASIC)2−/− compared with wild-type (WT) mice. A: representative smooth muscle α-actin immunofluorescence images of lung sections from control (Con) and chronic hypoxia (CH) WT, ASIC2−/−, and ASIC3−/− mice. Fluorescence images were digitally inverted to provide better contrast and visibility of immunofluorescence. B: %muscularization calculated as %thresholded smooth muscle α-actin area divided by total arterial wall area. All vessels were <100 µm. Values are means ± SE; n = no. of vessels (indicated in bar) analyzed from 4 animals/group. ****P ≤ 0.0001 vs. the control group; ####P < 0.0001 vs. corresponding WT mice.

Techniques: Immunofluorescence, Fluorescence

Effect of acid-sensing ion channel (ASIC) on baseline ventilation and blood gases following chronic hypoxia (CH). Whole body plethysmography was used to determine respiratory frequency (breaths/min; A), tidal volume (μl·breath−1·g body wt−1; B), and minute ventilation (ml·min−1·g body wt−1; C) in conscious wild-type (WT), ASIC2−/−, and ASIC3−/− CH mice breathing room air. Baseline arterial Po2 (mmHg; D), Pco2 (mmHg; E), and pH (F) in conscious WT, ASIC2−/−, and ASIC3−/− CH mice breathing room air. Values are means ± SE; n/group are indicated at the bottom of the bars as no. of males/females and as color-coded dots: black square, CH male; red square, CH female. There is no significant difference between males and females (P = 0.979). *P < 0.05 and **P < 0.01 vs. respective WT, analyzed by 1-way ANOVA and individual groups compared using Tukey’s multiple-comparisons test.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Effect of acid-sensing ion channel (ASIC) on baseline ventilation and blood gases following chronic hypoxia (CH). Whole body plethysmography was used to determine respiratory frequency (breaths/min; A), tidal volume (μl·breath−1·g body wt−1; B), and minute ventilation (ml·min−1·g body wt−1; C) in conscious wild-type (WT), ASIC2−/−, and ASIC3−/− CH mice breathing room air. Baseline arterial Po2 (mmHg; D), Pco2 (mmHg; E), and pH (F) in conscious WT, ASIC2−/−, and ASIC3−/− CH mice breathing room air. Values are means ± SE; n/group are indicated at the bottom of the bars as no. of males/females and as color-coded dots: black square, CH male; red square, CH female. There is no significant difference between males and females (P = 0.979). *P < 0.05 and **P < 0.01 vs. respective WT, analyzed by 1-way ANOVA and individual groups compared using Tukey’s multiple-comparisons test.

Techniques:

Blood gas measurements and ventilatory responses in response to hypoxia, isocapnic hypoxia, or hypercapnia in conscious, chronically catheterized WT, ASIC2 −/− , and ASIC3 −/− mice following 4 wk of CH

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Blood gas measurements and ventilatory responses in response to hypoxia, isocapnic hypoxia, or hypercapnia in conscious, chronically catheterized WT, ASIC2 −/− , and ASIC3 −/− mice following 4 wk of CH

Techniques:

Enhanced pulmonary vasoconstrictor reactivity in control acid-sensing ion channel (ASIC)2−/− and ASIC3−/− mice. Baseline pulmonary vascular resistance (PVR; in mmHg·ml−1·min·kg−1; A) and changes (Δ) in PVR in response to hypoxic ventilation (6% CO2 and balance N2; B), KCl (30 mM; C), and U-46619 (10−9 – 10−6 M; D) in isolated lungs from control wild-type (WT), ASIC2−/−, and ASIC3−/− mice. All experiments were conducted in the presence of NG-nitro-l-arginine (300 μM) and meclofenamate (30 μM). Values are means ± SE; n/group indicated as color-coded dots: black circle, control male; red circle, control female. **P < 0.01 and ***P < 0.001 vs. corresponding WT, analyzed by 1-way ANOVA and individual groups compared using Tukey’s multiple comparisons test. NS, not significant.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Enhanced pulmonary vasoconstrictor reactivity in control acid-sensing ion channel (ASIC)2−/− and ASIC3−/− mice. Baseline pulmonary vascular resistance (PVR; in mmHg·ml−1·min·kg−1; A) and changes (Δ) in PVR in response to hypoxic ventilation (6% CO2 and balance N2; B), KCl (30 mM; C), and U-46619 (10−9 – 10−6 M; D) in isolated lungs from control wild-type (WT), ASIC2−/−, and ASIC3−/− mice. All experiments were conducted in the presence of NG-nitro-l-arginine (300 μM) and meclofenamate (30 μM). Values are means ± SE; n/group indicated as color-coded dots: black circle, control male; red circle, control female. **P < 0.01 and ***P < 0.001 vs. corresponding WT, analyzed by 1-way ANOVA and individual groups compared using Tukey’s multiple comparisons test. NS, not significant.

Techniques: Isolation

Augmented control acid-sensing ion channel (ASIC)1-dependent basal Ca2+ and store-operated calcium entry (SOCE) in pulmonary arterial smooth muscle cells (PASMC) from ASIC2−/− mice. A: summary data showing basal and Ca2+ free [Ca2+]i levels (fura2 340/380 ratio) in PASMC from control wild-type (WT), ASIC2−/−, or ASIC3−/− male mice. B: [Ca2+]i levels (fura2 340/380 ratio) following treatment with diltiazem (50 μM) or PcTX1 (20 nM). C: SOCE responses performed in the presence of cyclopiazonic acid (CPA; 10 μM), diltiazem (50 μM), and ± PcTX1. Values are means ± SE; ●, n of 6–10/group. *P < 0.05 and ****P < 0.0001 vs. WT; #P < 0.05 and ####P < 0.0001 vs. corresponding baseline/vehicle, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple comparisons test.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Augmented control acid-sensing ion channel (ASIC)1-dependent basal Ca2+ and store-operated calcium entry (SOCE) in pulmonary arterial smooth muscle cells (PASMC) from ASIC2−/− mice. A: summary data showing basal and Ca2+ free [Ca2+]i levels (fura2 340/380 ratio) in PASMC from control wild-type (WT), ASIC2−/−, or ASIC3−/− male mice. B: [Ca2+]i levels (fura2 340/380 ratio) following treatment with diltiazem (50 μM) or PcTX1 (20 nM). C: SOCE responses performed in the presence of cyclopiazonic acid (CPA; 10 μM), diltiazem (50 μM), and ± PcTX1. Values are means ± SE; ●, n of 6–10/group. *P < 0.05 and ****P < 0.0001 vs. WT; #P < 0.05 and ####P < 0.0001 vs. corresponding baseline/vehicle, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple comparisons test.

Techniques:

Enhanced pulmonary vasoconstrictor reactivity in control acid-sensing ion channel (ASIC)2−/− mice is mediated by ASIC1. A and B: basal arterial tone (%Ca2+ free diameter; A) and basal intracellular Ca2+ concentration ([Ca2+]i; B) expressed as vessel wall fura-2 ratio (F340/F380) in isolated, pressurized, small pulmonary arteries from control wild-type (WT) and ASIC2−/− mice in the presence or absence of psalmotoxin 1 (PcTX1; 20 nM). C and D: %vasoconstriction (C) and changes (Δ) in vessel wall Ca2+ (Δ in fura-2 ratio) (D) in response to KCl (30 mM). E–H: %vasoconstriction (E) and change in vessel wall Ca2+ (G) to U-46619 (10−10 to 10−6 M). U-46619 dose response curves were analyzed by evaluating maximum effect (Emax; E and G) and logEC50 (F and H). Values are means ± SE; ●, n/group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corresponding WT; #P < 0.05, ##P < 0.01, and ###P < 0.001, and ####P < 0.0001 vs. corresponding vehicle (Veh) treated, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple comparisons test.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Enhanced pulmonary vasoconstrictor reactivity in control acid-sensing ion channel (ASIC)2−/− mice is mediated by ASIC1. A and B: basal arterial tone (%Ca2+ free diameter; A) and basal intracellular Ca2+ concentration ([Ca2+]i; B) expressed as vessel wall fura-2 ratio (F340/F380) in isolated, pressurized, small pulmonary arteries from control wild-type (WT) and ASIC2−/− mice in the presence or absence of psalmotoxin 1 (PcTX1; 20 nM). C and D: %vasoconstriction (C) and changes (Δ) in vessel wall Ca2+ (Δ in fura-2 ratio) (D) in response to KCl (30 mM). E–H: %vasoconstriction (E) and change in vessel wall Ca2+ (G) to U-46619 (10−10 to 10−6 M). U-46619 dose response curves were analyzed by evaluating maximum effect (Emax; E and G) and logEC50 (F and H). Values are means ± SE; ●, n/group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corresponding WT; #P < 0.05, ##P < 0.01, and ###P < 0.001, and ####P < 0.0001 vs. corresponding vehicle (Veh) treated, analyzed by 2-way ANOVA and individual groups compared using Tukey’s multiple comparisons test.

Techniques: Concentration Assay, Isolation

Effect of acid-sensing ion channel (ASIC)2−/− and ASIC3−/− on ASIC1 expression. A: summary data showing mRNA analysis by real-time quantitative PCR for ASIC1 in primary pulmonary arterial smooth muscle cells (PASMC) from control wild-type (WT), ASIC2−/−, and ASIC3−/− mice. ASIC1 expression was normalized to 18S rRNA as an internal control (2–ΔΔCT). B and C: representative Western blots for ASIC1 (indicated by arrow; B) and summary data for protein analysis of ASIC1 (normalized to entire lane on Coomassie-stained blot; C) in primary PASMC from WT, ASIC2−/−, and ASIC3−/− mice. Values are means ± SE. Nos. of animals/group (6, 7) are indicated as color-coded dots: black circle, control male; red circle, control female, analyzed by 1-way ANOVA.

Journal: Journal of Applied Physiology

Article Title: Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

doi: 10.1152/japplphysiol.00894.2018

Figure Lengend Snippet: Effect of acid-sensing ion channel (ASIC)2−/− and ASIC3−/− on ASIC1 expression. A: summary data showing mRNA analysis by real-time quantitative PCR for ASIC1 in primary pulmonary arterial smooth muscle cells (PASMC) from control wild-type (WT), ASIC2−/−, and ASIC3−/− mice. ASIC1 expression was normalized to 18S rRNA as an internal control (2–ΔΔCT). B and C: representative Western blots for ASIC1 (indicated by arrow; B) and summary data for protein analysis of ASIC1 (normalized to entire lane on Coomassie-stained blot; C) in primary PASMC from WT, ASIC2−/−, and ASIC3−/− mice. Values are means ± SE. Nos. of animals/group (6, 7) are indicated as color-coded dots: black circle, control male; red circle, control female, analyzed by 1-way ANOVA.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Staining

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rabbit anti asic2 (Alomone Labs)

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